. 2018; 34(1): 46-47 | DOI: 10.5152/eamr.2018.81300 | |||
Patolojide Floresan in situ HibridizasyonÖzben Yalçın, Gamze KuldukOkmeydanı Eğitim ve Araştırma Hastanesi, Patoloji Bölümü, İstanbulFloresan in situ hibridizasyon (FISH), floresan probların hücre çekirdeği üzerindeki belirli kromozamal bölgelere bağlanması ile kromozomal bozuklukların saptanmasını sağlayan moleküler sitogenetik bir tekniktir. Son yıllarda tümöre spesifik kromozomal translokasyonlar, delesyonlar, amplifikasyonlar ve yeni onkojenlerin saptanması ile FISH yöntemi diagnostik, terapödik ve prognostik açılardan tıp pratiğinde büyük önem kazanmıştır. Fluorescence in Situ Hybridization in PathologyÖzben Yalçın, Gamze KuldukDepartment of Pathology, University of Health Sciences, Okmeydanı Training and Research Hospital, İstanbul, TurkeyFluorescence in Situ Hybridization (FISH) is a molecular cytogenetic technique that uses fluorescent probes which bind to specific chromosomal locations within the nucleus to search and detect chromosomal abnormality. In the last decade, tumor-specific chromosomal translocations, deletions, gains, amplifications, and novel oncogenes have become increasingly important in the field of diagnostic, therapeutic, and prognostic concepts in medicine. FISH technique provides a quick analysis of formalin-fixed paraffin-embedded cells which can be used in daily practice of pathology for several tumor types. Fluorescence in Situ Hybridization (FISH) technique is based on hybridization of tagged DNA probes which are fluorescent reporter molecules that affirm the presence or absence of particular genetic anomaly under fluorescence microscopy. This technique has been recently developed to screen the whole genome coexistently through multicolor whole chromosome probe techniques that multiplex-FISH or spectral-karyotyping or through an array-based method using comparative genomic hybridization. The aim of this article was to provide a theoretical survey of FISH for clinical function in surgical pathology habit. Keywords: FISH, molecular genetics, pathologyÖzben Yalçın, Gamze Kulduk. Fluorescence in Situ Hybridization in Pathology. . 2018; 34(1): 46-47 Sorumlu Yazar: Özben Yalçın, Türkiye |
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