The Ameliorative Effects of Ethyl Pyruvate and Dimethyl Sulfoxide on Ischemic Tissue Injury in Experimental Carbon Monoxide IntoxicationSinan Pasli1, Melih Imamoglu2, Mustafa Çiçek3, Metin Yadigaroglu4, Aynur Sahin5, Yunus Karaca5, Esin Yulug6, Ozgur Tatli51Department of Emergency Medicine, Gümüşhane State Hospital, Gümüşhane, Turkey 2Department of Emergency Medicine, Rize State Hospital, Rize, Turkey 3Department of Emergency Medicine, Bayburt State Hospital, Bayburt, Turkey 4Department of Emergency Medicine, Fatih State Hospital, Trabzon, Turkey 5Department of Emergency Medicine, Karadeniz Technical University, Faculty of Medicine, Trabzon, Turkey 6Department of Histology and Embryology, Karadeniz Technical University, Faculty of Medicine, Trabzon, Turkey
Aim: The purpose of this study was to investigate the protective effects of ethyl pyruvate (EP) and dimethyl sulfoxide (DMSO) against ischemic tissue in brain, cardiac and hepatic tissue in experimentally induced carbon monoxide (CO) intoxication.
Material and Methods: Thirty-five mature female Sprague Dawley rats were randomized into five groups of seven animals each. Group-I received no CO or treatment. Rats in groups II,III,IV and V were made to inhale a high-concentration 5000 ppm CO gas mixture for 60 min at 4 L/min. CO levels were then measured from 1ml tail vein blood. Group-II received no therapeutic agent, Group-III received 6 mg/kg intraperitoneal (ip) DMSO, Group-IV received 50 mg/kg ip EP, Group-V received 50mg/kg EP and 6mg/kg DMSO ip. All rats were sacrificed by decapitation. Brain, cardiac and hepatic tissues were removed and histopathological scores were compared.
Results: Comparison of Group-II with Group-III and Group-II with Group-V revealed that DMSO alone and EP+DMSO exhibited a reducing effect on degree of cerebral neuronal alteration, degenerative neuron rates, and total cardiac injury score (p=0.005, p=0.002, p=0.004 and p=0.002, p=0.001, p=0.004 respectively). There was not any histopathological difference between Group-II and Group-IV suggesting EP alone has no therapeutic effect on histopathological injury.
Conclusion: Based on our study findings, EP administered alone exhibited no protective effects against the organ injuries investigated, while DMSO exhibited reducing effects on degree of neuron alteration, rates of degenerative neurons, and total cardiac injury scores. An ameliorating effect on cardiac and hepatic injury was more prominent with combined treatment. Keywords: carbon monoxide, dimethyl sulfoxide, ethyl pyruvate, experimental, intoxication
Sinan Pasli, Melih Imamoglu, Mustafa Çiçek, Metin Yadigaroglu, Aynur Sahin, Yunus Karaca, Esin Yulug, Ozgur Tatli. The Ameliorative Effects of Ethyl Pyruvate and Dimethyl Sulfoxide on Ischemic Tissue Injury in Experimental Carbon Monoxide Intoxication. . 9999; 0: 0-0
Corresponding Author: Sinan Pasli, Türkiye |
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