Investigation of expression of CYP3A4 in diabetic rats in xenobiotic metabolism

INTRODUCTION: This study aimed to investigate the impact of high-fat diet and STZ induced diabetes as well as dapagliflozin treatment on hepatic protein expression of CYP3A4.
METHODS: In our study, 34 male Sprague-Dawley rats were randomly divided into four groups: control, high-fat diet and streptozotocin induced diabetes, dapagliflozin treated control and dapagliflozin treated diabetes. In the microsomes obtained from livers of these rats, the protein expression levels of CYP3A4 were determined by Western blot.
RESULTS: Hepatic CYP3A4 protein expression levels in control group treated with dapagliflozin were statistically significantly decreased compared to control group. Besides, hepatic CYP3A4 protein expression levels were decreased in dapagliflozin treated diabetic Sprague-Dawley rats compared to both control and diabetic group rats, but the difference between the groups was not statistically significant.
DISCUSSION AND CONCLUSION: According to these two results, the use of dapagliflozin resulted in inhibition of hepatic CYP3A4 protein expression.