. 2017; 14(3): 0-0

Synchronous Fluorescence Spectrofluorımetrıc Method For The Sımultaneous Determınatıon Of Clonazepam And Paroxetıne Hydrochlorıde In Combıned Pharmaceutıcal Dosage Form

Jalpa U. Patel, Usmangani K. Chhalotiya
Indukaka Ipcowala College of Pharmacy, Beyond GIDC, P.B. No. 53, Vitthal Udyognagar- 388 121, Gujarat, India

A novel synchronous fluorescence method is described for simultaneous analysis of binary mixture of Clonazepam (CLO) and Paroxetine hydrochloride (PH) in combined tablet dosage form without any prior separation of components from the sample. CLO was determined at emission wavelength of 512.79 nm (zero-crossing wavelength point of PH). Similarly, PH was measured at 336.00 nm (zero-crossing wavelength point of CLO). The first derivative amplitude- concentration plots were rectilinear over the range of 1-5 μg/mL for CLO and 5-25 μg/mL for PH. The method was validated statistically as per ICH guidelines. The limits of detection were 0.055 and 0.033 μg/mL and quantification limits were 0.169 and 0.102 μg/mL for CLO and PH, respectively. The % recovery in commercial formulation was found to be in the range 100.45 % and 99.38 % for CLO and PH, respectively by the proposed method and % RSD values for precision and accuracy studies were found to be less than 2. The proposed method can be successfully applied for routine analysis of CLO and PH in combined dosage form.

Keywords: First derivative synchronous spectrofluorimetry, Clonazepam, Paroxetine hydrochloride


Synchronous Fluorescence Spectrofluorımetrıc Method For The Sımultaneous Determınatıon Of Clonazepam And Paroxetıne Hydrochlorıde In Combıned Pharmaceutıcal Dosage Form

Jalpa U. Patel, Usmangani K. Chhalotiya
Indukaka Ipcowala College of Pharmacy, Beyond GIDC, P.B. No. 53, Vitthal Udyognagar- 388 121, Gujarat, India

A novel synchronous fluorescence method is described for simultaneous analysis of binary mixture of Clonazepam (CLO) and Paroxetine hydrochloride (PH) in combined tablet dosage form without any prior separation of components from the sample. CLO was determined at emission wavelength of 512.79 nm (zero-crossing wavelength point of PH). Similarly, PH was measured at 336.00 nm (zero-crossing wavelength point of CLO). The first derivative amplitude- concentration plots were rectilinear over the range of 1-5 μg/mL for CLO and 5-25 μg/mL for PH. The method was validated statistically as per ICH guidelines. The limits of detection were 0.055 and 0.033 μg/mL and quantification limits were 0.169 and 0.102 μg/mL for CLO and PH, respectively. The % recovery in commercial formulation was found to be in the range 100.45 % and 99.38 % for CLO and PH, respectively by the proposed method and % RSD values for precision and accuracy studies were found to be less than 2. The proposed method can be successfully applied for routine analysis of CLO and PH in combined dosage form.

Anahtar Kelimeler: First derivative synchronous spectrofluorimetry, Clonazepam, Paroxetine hydrochloride


Jalpa U. Patel, Usmangani K. Chhalotiya. Synchronous Fluorescence Spectrofluorımetrıc Method For The Sımultaneous Determınatıon Of Clonazepam And Paroxetıne Hydrochlorıde In Combıned Pharmaceutıcal Dosage Form. . 2017; 14(3): 0-0

Corresponding Author: Jalpa U. Patel, India


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