Various in vitro bioactivities of secondary metabolites isolated from the sponge Hyrtios aff. erectus from the Red Sea coast of Egypt

INTRODUCTION: The present study confirmed the presence of bioactive constituents in Hyrtio saff.erectus Sponge extract (HESE) collected from Red Sea.
METHODS: The current study subjected to broad biochemical assays for example cytotoxicity, antioxidants (DPPH and ABTS),anti-inflammatory (IL-6, TNF-α,COX-1and COX-2,anticancer (PTK and SHK1) anti-Alzheimer using acetylcholinesterase.Furthermore different phytochemical screening.
RESULTS: The Cytotoxicity was tested against hepatocellular carcinoma cell lines as prescreening test. The HES extract had high content of total phenolic, flavonoids and carotenoid with 0.061, 0.2839 and 1.976 mg/g; respectively.The pharmacological and biochemical studies showed high antioxidant capacity with 93.0% and 99% at 1mg using DPPH and ABTS respectively. Cytotoxic activity against cancerous cell line cleared out that HES could inhibit cell growth effectively with IC50 = 47.5 µg/ml. Furthermore, anticancer activity using tyrosine kinase (PTK) and Sphingosine kinase 1(SHKI) inhibitor screening assays resulted in 71.66 and 85.21% inanition activity; respectively. The anti-inflammatory assays showed that the inhabitation activities against COX-1, COX-2, IL-6 and TNF-α were 71.82, 81.13, 80.89 and 59.74 %; respectively.At the same time, the anti-Alzheimer results showed high activity at 1mg with 83.51 %. Additionally the antiviral activities using reverse transcriptase inanition assay was 91.70%.
DISCUSSION AND CONCLUSION: The Hyrtio saff erectus sponge isolated from red sea showed tremendous activity against many diseases and it is consider as an excellent source for bioactive pharmaceutical compounds.