Pharmacological potential effects of Algerian propolis against oxidative stress, multi-drug resistant pathogens biofilm and quorum-sensing
Widad Hadjab1, amar zellagui1, Meryem Mokrani1, Mehmet Öztürk2, Ozgur Ceylan2, Noureddine Gherraf3, chawki bensouici41Laboratory of Biomolecules and Plant Breeding, Life Science and Nature Department, Faculty of Exact Science and Life Science and Nature, University of Larbi Ben MhidiOum El Bouaghi, Algeria2Food Quality and Analysis Program, Ula Ali Kocman Vocational School, Mugla Sitki Kocman University, 48000 Mugla, Turkey
3Laboratory Of Natural Resources And Management Of Sensitive Environments, University Of Larbi Ben M’hidi, Oum El Bouaghi, Algeria
4Biotechnology Research Centre, Constantine 25000, Algeria
INTRODUCTION: This study sought to examine the chemical profile, antioxidant, antimicrobial, antibiofilm, and anti-quorum sensing potential of two propolis ethanolic extracts collected from Northeast Algeria.
METHODS: To reach the main objectives of this study, multiple in vitro tests were employed. The phenolic and flavonoid content were analyzed, and the chemical composition of both propolis ethanolic extracts was determined by High-performance liquid chromatography analysis. The investigation of the antioxidant properties of the propolis extracts was determined using six complementary tests. The inhibitory effects of propolis extracts were performed against multidrug-resistant clinical isolates using agar well diffusion and micro-dilution methods, while antibiofilm and quorum sensing disruption effects were determined by spectrophotometric microplate methods.
RESULTS: The results demonstrated that phenolic and flavonoid contents were higher in propolis from Guelma region (PEEG; 188.50±0.33 μg GAE/mg E, 144,23± 1.03 μg QE/mg E), respectively. Interestingly, different components were identified and cynarin was the major compound detected. The PEEG sample exhibited potential antioxidant effects in scavenging ABTS•+ radicals with minimal IC50 values equal to 10.46±1.40µg/ml. Furthermore, the highest antibacterial activity was recorded by PEEG against Gram-positive Staphylococcus aureus MDR1. Similarly, the PEEG effectively inhibited the biofilm formation of S. aureus MDR1and the degradation of biofilm was up to 60%. In addition, Quorum sensing disruption revealed that both extracts have a moderate capacity for violacein inhibition by Chromobacterium violaceum ATCC 12472 strain in a concentration-dependent manner.
DISCUSSION AND CONCLUSION: These findings indicated that propolis can be regarded as a natural therapeutic agent for health problems associated with Multidrug resistant bacteria and oxidative stress
Corresponding Author: amar zellagui, Algeria
| TOOLS |
 Print Download citation RIS EndNote BibTex Medlars Procite Reference Manager Share with email Share Send email to author
Similar articles Google Scholar |