Degradation kinetics, in-vitro dissolution studies and quantification of praziquantel anchored in emission intensity by spectrofluorimetry

INTRODUCTION: A simple, rapid, specific and highly sensitive ecofriendly spectrofluorimetric method has been developed for the quantification of praziquantel.
METHODS: A linear relationship was found between fluorescence intensity and praziquantel concentration in the range of 1-20 µg/mL in water at emission wavelength of 286 nm after excitation wavelength at 263 nm with a good correlation coefficient (0.999).
RESULTS: The proposed method was validated according to ICH guidelines and statistical analysis of the results revealed high accuracy and good precision with the percentage RSD values less than 2.The detection and quantification limits were found to be 0.27 and 0.81 µg/mL, respectively.The proposed method was extended to investigate the stability of the drug and its degradation kinetics in the presence of acidic, alkaline and oxidative conditions.
DISCUSSION AND CONCLUSION: Moreover, the method was utilized to study the in-vitro dissolution studies of praziquantel tablet formulation.The suggested procedures could be used for the assessment of praziquantel in drug substance and drug products as well as in presence of its degradation products.