In Vitro Macrophage Nitric Oxide and Interleukin-1 Beta Suppression by Moringa Peregrina SeedShaymaa Fadhel Abbas Albaayit, Ahmed Salim Kadhim Al-Khafaji, Hiba Sarmed AlnaimyDepartment of Biology, Faculty of Science, University of Baghdad, Baghdad-Iraq
INTRODUCTION: Moringa peregrina have long been used in folk medicine to treat diseases including fever, headache, burns, constipation, gut pains and inflammatory. Nitric oxide (NO) and interleukin-1 beta (IL-1β) play an important role in the pathophysiology of inflammation. The objectives of this study were to determine the effect of Moringa peregrina seed ethanolic extract (MPSE) on the viability of macrophages and NO and IL-1β production by lipopolysaccharide (LPS)-activated macrophage (J774A.1) cell line. METHODS: The 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) assay was used to determine the cytotoxic effect of MPSE treatment at concentrations ranging from 31.15 to 1000 µg/ml. The Nitric oxide concentration was determined by Griess assay and interleukin-1 beta proinflammatory cytokine concentration by enzyme-linked immunosorbent assay (ELISA) in the supernatant of Moringa peregrina seed ethanolic extract -treated lipopolysaccharide -activated J774A.1 cell culture. The statistical values were determined employing the statistical test of one-way analysis of variance. RESULTS: Results: The results show that the Moringa peregrina seed ethanolic extract was not cytotoxic at 1000 μg/ml while significantly (p<0.001) inhibited Nitric oxide and interleukin-1 beta production by the lipopolysaccharide -activated macrophage J774A.1 cells.
DISCUSSION AND CONCLUSION: Conclusion: These findings suggest that the Moringa peregrina seed extract can be used to treat and prevent inflammatory diseases through the inhibition of inflammatory mediators. In spite of the fact that our findings was obtained by conducting an experimental research study, the outcomes, however, is promising and paving the way for foreseeable clinical prospective study.
Keywords: Moringa peregrina, nitric oxide, interleukin-1β,, inflammation
Shaymaa Fadhel Abbas Albaayit, Ahmed Salim Kadhim Al-Khafaji, Hiba Sarmed Alnaimy. In Vitro Macrophage Nitric Oxide and Interleukin-1 Beta Suppression by Moringa Peregrina Seed. . 2019; 16(3): 362-365
Corresponding Author: Shaymaa Fadhel Abbas Albaayit, Iraq |
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