Lc-Dad-Ms-Assisted Quantification Of Marker Compounds In Hypericum Perforatum L. (St. John’s Wort) And Its Antioxidant Activity

Erdoğan Orhan, İlkay; Kartal, Murat; Kartal, Murat

İlkay Erdoğan Orhan¹, Murat Kartal², Murat Kartal³

¹Department Of Pharmacognosy, Faculty Of Pharmacy, Gazi University, 06330 Ankara, Turkey
²Department Of Pharmacognosy, Faculty Of Pharmacy, Ankara University, 06100 Ankara, Turkey
³Department Of Pharmacognosy, Faculty Of Pharmacy, And Center Of Phytotherapy Education, Research And Practice, Bezmialem Vakif University, 34093 Istanbul, Turkey

Hypericum perforatum L. (St. John’s Wort) is a reputed plant with a long service to humankind. In the current study, antioxidant activity of the methanol extract of the aerial parts of H. perforatum growing in Turkey along with hyperoside and hyperforin was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, metal-chelation, and ferric-reducing antioxidant power (FRAP) assays. The major components including chlorogenic acid, the flavonoid derivatives; rutin, hyperoside, quercitrin, quercetin, and biapigenin, the naphthodianthrons; pseudohypericin and hypericin, and the phloroglucinol derivatives; hyperforin and adhyperforin were quantified in the extract by LC-DAD-MS. Hypericin (16 ± 0.08 μg g-1) and hiperforin (1164 ± 0.02 μg g-1) contents in H. perforatum were found to be in accordance with the amounts required by the European Pharmacopeia. The extract and hyperoside exerted a remarkable antioxidant activity in DPPH radical scavenging and FRAP assays, whereas they did not have metal-chelation capacity.

Keywords:

Hypericum perforatum L.’da (St. John’s Wort) İşaretleyici Maddelerin LC-DAD-MS ile Miktar Tayini ve Antioksidan Aktivitesi

İlkay Erdoğan Orhan¹, Murat Kartal², Murat Kartal³

Hypericum perforatum L. (St. John’s Wort) insanoğluna uzun süredir faydalı olan ünlü bir bitkidir. Mevcut çalışmada, Türkiye’de yetişen H. perforatum’un topraküstü kısımlarından elde edilen metanol ekstresi ile hiperozit ve hiperforin’in antioksidan aktivitesi, 2,2-difenil-1-pikrilhidrazil (DPPH) radikal süpürücü, metal-şelasyon ve demir-indirgeme antioksidan gücü (FRAP) yöntemleriyle değerlendirilmiştir. Ekstrede, klorojenik asit, flavonoit türevleri olan rutin, hiperozit, kersitrin, kersetin ve biapigenin, naftodiantron türevleri olan psödohiperisin ve hiperisin ile floroglusinol türevleri olan hiperforin ve adhiperforin miktarları LC-DAD-MS ile tayin edilmiştir. H. perforatum’daki hiperisin (16 ± 0.08 μg g-1) ve hiperforin (1164 ± 0.02 μg g-1) içerikleri, Avrupa Farmakopesi tarafından istenen miktarlara uygun bulunmuştur. Ekstre ve hiperozit DPPH radikal süpürücü ve FRAP yöntemlerinde dikkate değer bir antioksidan aktivite gösterirken, metal-şelasyon kapasiteye sahip olmadıkları bulunmuştur.

Anahtar Kelimeler:

İlkay Erdoğan Orhan, Murat Kartal, Murat Kartal. Lc-Dad-Ms-Assisted Quantification Of Marker Compounds In Hypericum Perforatum L. (St. John’s Wort) And Its Antioxidant Activity. . 2015; 12(3): 30-39

Corresponding Author: İlkay Erdoğan Orhan, Türkiye