. 2018; 15(3): 0-0

Formulation and optimization of gentamicin hydrogel infused with tetracarpidium conophorum extract via Central composite design for topical delivery

Margaret Okonawan Ilomuanya1, Nosakhare Andrew Amenaghawon2, Joy Odimegwu3, Omotunde Olufunke Okubanjo1, Chinelo Aghaizu1, Adeyinka Oluwatobiloba1, Thomas Akimien1, Tolulope Ajayi1
1Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy. University of Lagos, PMB 12003, Surulere, Lagos, Nigeria.
2Department of Chemical Engineering, Faculty of Engineering, University of Benin, Benin, Edo State
3Department of Pharmacognosy, Faculty of Pharmacy. University of Lagos, PMB 12003, Surulere, Lagos, Nigeria

INTRODUCTION: Response surface methodology coupled with statistically designed experiment has been found to be very useful in optimising multivariable processes. The aim of this study is to evaluate the influence of two independent variables, a ratio of permeation enhancers/antioxidants (Transcutol and ethanolic extract of Tetracarpidium conophorum EETC) and stirring rate, on the flux and permeation of gentamicin hydrogel.
METHODS: A modification of free radical initial polymerization was utilized to formulate the gentamicin hydrogel. A 3² factorial central composite design (CCD) was then utilized to investigate the effect of independent variables of permeation enhancer Transcutol: EETC (X1), Stirring speed (X2) via 14 formulation batches which were evaluated for dependent variables Flux (Y1) and amount of drug permeated after 12 hours (Y2) ex-vivo.
RESULTS: The results of analysis of variance (ANOVA) carried out to determine the fit of the models revealed that the models were statistically significant (p < 0.05) and did not show lack of fit (R2 > 0.80). The regression equation generated for flux was Y1 = 19.35 – 25.82X1 – 0.044X2 + 0.0097X1X2 + 11.86X21 and for cumulative permeation of gentamicin in 12 hours Y2= 315.50 – 189.67X1 + 0.28X2 -1.29X1X2 + 123.55X21. The validity of the statistical models used for predicting flux and drug permeation was confirmed by carrying out three confirmation experimental runs at the identified optimum conditions. The results showed that there was no significant difference between the experimental results and those predicted by the statistical models.
DISCUSSION AND CONCLUSION: The excellent correlation between the predicted and measured values shows the validity of statistical models (R2= 0.95). Utilization of antioxidant and permeation enhancer has been used for the first time to investigate the influence on dependent variables. Optimization of gentamicin hydrogel using central composite statistical design is valid for prediction of drug permeation and flux utilizing variables in formulation.

Keywords: Hydrogels, polymer, Tetracarpidium conophorum, Response surface methodology, gentamicin


Margaret Okonawan Ilomuanya, Nosakhare Andrew Amenaghawon, Joy Odimegwu, Omotunde Olufunke Okubanjo, Chinelo Aghaizu, Adeyinka Oluwatobiloba, Thomas Akimien, Tolulope Ajayi. Formulation and optimization of gentamicin hydrogel infused with tetracarpidium conophorum extract via Central composite design for topical delivery. . 2018; 15(3): 0-0

Corresponding Author: Margaret Okonawan Ilomuanya, Nigeria


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