Effect of Zn, Fe And Cu Content On Phytochemical Investigations and Antimicrobial Potentưal of Alternanthera Brasiliana(L.) O. Kuntze Leaf Extracts Procured From Two Different States of India
Kuntal Das1, Sourav Tribedi21Krupanidhi College Of Pharmacy, #12/1, Carmelaram Post, Varthur Hobli. Bangalore-35,ưndia2Gautham College Of Pharmacy, Sulthanpalya, R. T. Nagar Post. Bangalore-560032. India
The effect of zinc (Zn), iron (Fe) and copper (Cu)on phytoconstituent content and the potential antibacterial activity ofthree different leaf extracts of Alternantherabrasiliana(AB) against pathogenic bacteria, procured fromtwo different states of India, was evaluated. Shade dried leaf samples were separately determined for Zn, Fe and Cu content through atomic absorption spectrophotometer (AAS). Furtherextractionwas carried out using aqueous, methanol and mixture of aqueous and methanol (1: 1) and microbiosidepotential were evaluated by agar well diffusion technique. Ampicillin (30µg/ml) was used as standard. The Minimum Inhibition Concentration (MIC) of all the extracts was carried out by serial dilution method and thereafter inhibitory microbial activity was carried out by agar plate method. The amount of Zn, Fe and Cu were higher in West Bengal sample. Furthermore, the concentration dependent (**P<0.05) potential antimicrobial activity was observed. At the dose of 200 mg/ml,combined aqueous and methanol extract of AB (ABAE and ABME respectively) from West Bengal, gave significant results against gram positive bacteria where maximum zone of inhibition was recorded against Straphyloccusaureus(13.0± 0.011**).Such variation may be due to the geographical location of the plant, soil pH, content of elements and on theeffects of choice of solvent.
Keywords: Antimicrobial study, agar diffusion technique, elemental analysis, extracts, Alternantherabrasiliana, MIC.Effect of Zn, Fe And Cu Content On Phytochemical Investigations and Antimicrobial Potentưal of Alternanthera Brasiliana(L.) O. Kuntze Leaf Extracts Procured From Two Different States of India
Kuntal Das1, Sourav Tribedi21Krupanidhi College Of Pharmacy, #12/1, Carmelaram Post, Varthur Hobli. Bangalore-35,ưndia2Gautham College Of Pharmacy, Sulthanpalya, R. T. Nagar Post. Bangalore-560032. India
The effect of zinc (Zn), iron (Fe) and copper (Cu)on phytoconstituent content and the potential antibacterial activity ofthree different leaf extracts of Alternantherabrasiliana(AB) against pathogenic bacteria, procured fromtwo different states of India, was evaluated. Shade dried leaf samples were separately determined for Zn, Fe and Cu content through atomic absorption spectrophotometer (AAS). Furtherextractionwas carried out using aqueous, methanol and mixture of aqueous and methanol (1: 1) and microbiosidepotential were evaluated by agar well diffusion technique. Ampicillin (30µg/ml) was used as standard. The Minimum Inhibition Concentration (MIC) of all the extracts was carried out by serial dilution method and thereafter inhibitory microbial activity was carried out by agar plate method. The amount of Zn, Fe and Cu were higher in West Bengal sample. Furthermore, the concentration dependent (**P<0.05) potential antimicrobial activity was observed. At the dose of 200 mg/ml,combined aqueous and methanol extract of AB (ABAE and ABME respectively) from West Bengal, gave significant results against gram positive bacteria where maximum zone of inhibition was recorded against Straphyloccusaureus(13.0± 0.011**).Such variation may be due to the geographical location of the plant, soil pH, content of elements and on theeffects of choice of solvent.
Anahtar Kelimeler: Antimicrobial study, agar diffusion technique, elemental analysis, extracts, Alternantherabrasiliana, MIC.Corresponding Author: Kuntal Das, India
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