. 2019; 16(2): 0-0

Preparation and Biopharmaceutical Evaluation of Novel Polymeric Nanoparticles Containing Etoposide for targeting the cancer cells

Ayyappan Thiyagarajan
Department of Pharmaceutics, Adhiparasakthi College of Pharmacy, Melmaruvathur- 603 319, Tamilnadu, India

INTRODUCTION: Polymeric nanoparticles is a promising novel drug delivery system and have advantageous in cancer therapy. Etoposide is an anticancer agent that is used in the treatment of a variety of malignancies. The present study was aimed to prepare and evaluate the Novel polymeric nanoparticles containing etoposide.
METHODS: 32 full factorial design was used to study the effect of Eudragit EPO and Pluronic F-68 on characterization of nanoparticles suspension. The polymeric nanoparticles was prepared by nano-precipitation technique. The prepared nanoparticles was evaluated by percentage yield, drug polymer compatibility using FTIR and DSC analysis, drug content, entrapment efficiency, zeta potential, particle size, SEM, XRD, In-vitro drug release studies, kinetic modeling, stability studies and in-vivo animal study. Response surface plots were studied which was generated using PCP dissolution software.
RESULTS: Scanning electron microscopic studies confirmed their porous structure with number of nanochannels. The FTIR spectra showed stable character of etoposide in mixture of polymers and revealed the absence of drug polymer interactions. DSC study revealed that drug was involved in complexation with nanoparticles. The average particle size of etoposide nanoparticles was found to be in the range of 114.4 nm to 136.7 nm. The results of zeta potential values were attained to ensure a good stability of nanosuspensions. In-vitro release of drug from nanoparticles follows peppas and showed controlled release behavior for a period of 24 h. The optimized nanoparticles were subjected to stability studies at 4șC in refrigerator and found most suitable temperature for storage of Etoposide nanoparticles. The average targeting efficiency of drug loaded nanoparticles was found to be 41.88 ±0.030% of the injected dose in liver, 25.66±0.320% in spleen 13.82±0.090% in lungs, 4.52±0.300% in kidney and 4.18±0.490% in brain.
DISCUSSION AND CONCLUSION: The study concluded that etoposide nanoparticles could be effective in sustained release and the drug loaded nanoparticles.

Keywords: Etoposide, Eudragit EPO, Pluronic F-68, 32 full factorial design, Nanoparticles.


Ayyappan Thiyagarajan. Preparation and Biopharmaceutical Evaluation of Novel Polymeric Nanoparticles Containing Etoposide for targeting the cancer cells. . 2019; 16(2): 0-0

Corresponding Author: Ayyappan Thiyagarajan, India


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