Microanatomical, Physicochemical characterization, and antioxidative activity of methanolic extract of Oudemansiella canarii (Jungh.) HöhnKrishnendu Acharya, Sudeshna Nandi, Arun Kumar DuttaDepartment Of Botany, University Of Calcutta, Kolkata, West Bengal, India
INTRODUCTION: Oudemansiella canarii, an edible mushroom highly appreciated throughout the world due to its gastronomic delicacy, is admiringly cultivated. Till date, no extensive work has been reported on the pharmacological and antioxidative aspect of this macrofungus. The present study focuses on the micro-morphological features, confirmation of its identity based on molecular sequence (nrITS rDNA) data, and physio-chemical parameters determination such as organoleptic features and fluorescent behaviour. METHODS: Collected basidiocarps were powdered and used for microscopic and organoleptic evaluation. DPPH radical scavenging method, total antioxidant activity methods and ABTS assay were used for evaluating antioxidant capacities of the methanolic extract. HPLC profile was also recorded to analyse phenolic fingerprint. RESULTS: The DPPH radical scavenging activity was determined with EC50 value of 0.912 µg, total antioxidant activity found to be 15.33 µg ascorbic acid equivalent/mg of extract and ABTS assay revealed 12.91 µm TE/mg of extract antioxidant activity. HPLC chromatogram revealed the presence of 12 peaks. Several parameters were tested for the determination of chemical composition that revealed the existence of major bioactive components in the extract in following order: phenol> flavanoid> ascorbic acid> β-carotene~ lycopene. DISCUSSION AND CONCLUSION: The present work suggests that Oudemansiella canarii may open a novel prospect as a functional food and antioxidant supplement.
Keywords: chromatographic fingerprinting, edible mushroom, fluorescence analysis, internal transcribed spacer, phytochemicals, West Bengal
Krishnendu Acharya, Sudeshna Nandi, Arun Kumar Dutta. Microanatomical, Physicochemical characterization, and antioxidative activity of methanolic extract of Oudemansiella canarii (Jungh.) Höhn. . 2019; 16(1): 0-0
Corresponding Author: Krishnendu Acharya, India |
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