. 2017; 14(1): 0-0 | DOI: 10.5505/tjps.2017.08108  

Adenosine A2B Receptors-Mediated Induction Of Interleukin-6 In Skeletal Muscle Cells

Mansour Haddad
Department of Clinical Sciences, Faculty of Pharmacy, Philadelphia University, Jordan

Inflammatory response and cytokine activation are markedly stimulated in skeletal muscle during various conditions. Interleukin-6 (IL-6), a pro-inflammatory cytokine, has pleiotropic effects on skeletal muscle. Adenosine, released by all cell types, binds to a class of G protein-coupled receptors to induce various skeletal muscle effects. The aim of this work was to investigate whether activation of adenosine receptors, particularly adenosine A2B receptors, could stimulate IL-6 gene expression in rat L6 skeletal muscle cells. The rat L6 skeletal muscle cells were cultured in 25 cm2 flasks. These differentiated cells were treated and then QRT-PCR (Probe-based) was used to analyze IL-6 gene expression level among different treatment conditions. Adenosine-5′-N-ethyluronamide (NECA), a stable adenosine analogue, concentration- and time-dependently stimulates IL-6 gene expression in skeletal muscle cells. The effect of NECA is inhibited by a selective adenosine A2B receptors antagonist, PSB 603. By using cAMP-arising reagent Forskolin, cAMP is found to be involved in the up-regulation of IL-6 induction. It has been demonstrated, for the first time, a novel relationship between adenosine and IL-6 up-regulation, in that IL-6 up-regulation induces by NECA is mediated by adenosine A2B receptor activation in skeletal muscle and is dependent on mainly a cAMP pathway. Adenosine A2B receptors are, thus, potentially important pharmacological targets in treating inflammation and related diseases in skeletal muscle tissues.

Keywords: Adenosine A2B receptors, skeletal muscle, interleukin 6, cAMP and inflammation


Adenosine A2B Receptors-Mediated Induction Of Interleukin-6 In Skeletal Muscle Cells

Mansour Haddad
Department of Clinical Sciences, Faculty of Pharmacy, Philadelphia University, Jordan

Inflammatory response and cytokine activation are markedly stimulated in skeletal muscle during various conditions. Interleukin-6 (IL-6), a pro-inflammatory cytokine, has pleiotropic effects on skeletal muscle. Adenosine, released by all cell types, binds to a class of G protein-coupled receptors to induce various skeletal muscle effects. The aim of this work was to investigate whether activation of adenosine receptors, particularly adenosine A2B receptors, could stimulate IL-6 gene expression in rat L6 skeletal muscle cells. The rat L6 skeletal muscle cells were cultured in 25 cm2 flasks. These differentiated cells were treated and then QRT-PCR (Probe-based) was used to analyze IL-6 gene expression level among different treatment conditions. Adenosine-5′-N-ethyluronamide (NECA), a stable adenosine analogue, concentration- and time-dependently stimulates IL-6 gene expression in skeletal muscle cells. The effect of NECA is inhibited by a selective adenosine A2B receptors antagonist, PSB 603. By using cAMP-arising reagent Forskolin, cAMP is found to be involved in the up-regulation of IL-6 induction. It has been demonstrated, for the first time, a novel relationship between adenosine and IL-6 up-regulation, in that IL-6 up-regulation induces by NECA is mediated by adenosine A2B receptor activation in skeletal muscle and is dependent on mainly a cAMP pathway. Adenosine A2B receptors are, thus, potentially important pharmacological targets in treating inflammation and related diseases in skeletal muscle tissues.

Anahtar Kelimeler: Adenosine A2B receptors, skeletal muscle, interleukin 6, cAMP and inflammation


Mansour Haddad. Adenosine A2B Receptors-Mediated Induction Of Interleukin-6 In Skeletal Muscle Cells. . 2017; 14(1): 0-0

Corresponding Author: Mansour Haddad, Jordan


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