Impact of particle-size reduction on solubility and antidiabetic activity of extracts of leaves of Vinca roseaKhalid Hussain, Abida Qamar, Nadeem Irfan Bukhari, Amjad Hussain, Naureen Shehzadi, Shaista Qamar, Sajida ParveenPunjab University College of Pharmacy, University of the Punjab, Lahore-54000, Pakistan
INTRODUCTION: The present study aimed to enhance aqueous-solubility of methanol extract of leaves of Vinca rosea (Family: Apocynaceae) by particle-size reduction using milling, and evaluate its antidiabetic activity. METHODS: The methanol extract (ME) was micronized using Vibratory Ball Mill, operated at vibratory speed of 15 Hz for 60 min, room temperature, and the resulting extract (MME) was investigated to determine particle-size, solubility, UV/Visible profile and in vitro antidiabetic activity. RESULTS: The average particle-size of MME was found to be 0.753±0.227 µm which was less than half of the ME (2.007±0.965 µm). The solubility of MME was found to be greater than the ME. MME exhibited 65.63, 18.0 and 96.87% higher antidiabetic activity in the glucose uptake by yeast cells method, hemoglobin glycosylation assay and the alpha amylase inhibition assay, respectively (p < 0.05).
DISCUSSION AND CONCLUSION: The results of the present study indicate that micronization has effectively enhanced the aqueous solubility and antidiabetic activity of methanol extract of leaves of Vinca rosea.
Keywords: Vinca rosea, Methanol extract, Micronization, Solubility, Antidiabetic activity
Khalid Hussain, Abida Qamar, Nadeem Irfan Bukhari, Amjad Hussain, Naureen Shehzadi, Shaista Qamar, Sajida Parveen. Impact of particle-size reduction on solubility and antidiabetic activity of extracts of leaves of Vinca rosea. . 2019; 16(3): 335-339
Corresponding Author: Khalid Hussain, Pakistan |
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