The Effect Of Human Umbilical Cord Stem Cells (Humsc) Administration On The Collagen Expression Of Anterior Vaginal Wall In Menopause Rats

Objective: to determine the effect of HUMSC administration on collagen expression in the anterior vaginal wall in menopause rats.
Materials and Methods: we conduct an experimental study with a randomized post-test only controlled group design. The samples were 40 female Winstar rat with inclusion criteria were healthy female Winstar rat that had been oovoriectomized, had never mated, aged 8 - 12 weeks, and weighed 18 - 22 grams. The umbilical cord was obtained from a voluntary donor who do not have a history of hepatitis B, hepatitis C, HIV, cytomegalovirus infection, treponema pallidum infection, or a history of other infections transmitted through the blood, placental tract and genitals. Data collection (anterior vaginal wall of the rat) is carried out in a controlled and controlled environment with the consideration that all conditions are maintained equally and can be controlled.
Results: There were 36 samples. A total of 13 menopausal rat (72%) had strong Collagen expression and 5 rat had weak-moderate Collagen expression (28%). In contrast, in the control group, 18 menopausal rat (100%) had weak-moderate Collagen expression and no menopausal rat appeared to have strong expression (0%). The administration of collagen to the anterior vaginal wall of postmenopausal rats can significantly increase the strong expression of collagen in the damaged anterior vagina of postmenopausal female rats (p<0.05).
Conclusion: Administration of HUMSC was proven to result in an increase in collagen levels in the anterior vaginal tissue of postmenopausal female rat. These results demonstrate significant therapeutic potential in the treatment of PFD.