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Fitoöstrojenlerin kırık iyileşmesi üzerine etkileri: Yeni Zelanda tavşanlarında deneysel çalışmaAlpaslan Öztürk1, Aysu Altıkardeşler İlman2, Hüsniye Sağlam3, Ulviye Yalçınkaya4, Serkan Aykut1, Semra Akgöz5, Yüksel Özkan1, Kemal Yanık2, Bijen Kıvçak3, Nazan Yalçın1, Recai Mehmet Özdemir11Bursa Yüksek İhtisas Eğitim Ve Araştırma Hastanesi, Ortopedi Ve Travmatoloji Kliniği, Bursa2Uludağ Üniversitesi Veterinerlik Fakültesi, Cerrahi Ana Bilim Dalı, Bursa 3Ege Üniversitesi Eczacılık Fakültesi, İzmir 4Uludağ Üniversitesi Tıp Fakültesi, Patoloji Ana Bilim Dalı, Bursa 5Uludağ Üniversitesi Tıp Fakültesi, Biyoistatistik Ana Bilim Dalı, Bursa AMAÇ: Fitoöstrojenler bitkisel kaynaklı doğal moleküller olup kemik yapımını artırıcı ve kemik yerine kullanılabilme özellikleri vardır. Bu çalışmada, fitoöstrojenlerin tavşan kırık modelinde kırık iyileşmesi üzerine etkileri deneysel olarak araştırıldı. GEREÇ-YÖNTEM: Yirmi iki adet Yeni Zelanda beyaz tavşanı sağ tibia kırığı oluşturulduktan sonra randomize olarak iki gruba ayrıldı. Birinci gruba Vitex agnus-castus L.’den (Verbenaceae) çalışma öncesinde hazırlanan bitki ekstresi intramuskuler olarak enjekte edildi. İkinci grup kontrol grubu olarak seçildi. Kırık iyileşmesi haftalık olarak kan alkalen fosfataz düzeyi, direkt radyografi ve 99m-Tc MDP kemik sintigrafisi ile değerlendirildi. Üçüncü haftanın sonunda çalışma sonlandırıldı. Çalışma sonunda histopatolojik inceleme yapıldı. BULGULAR: The effects of phytoestrogens on fracture healing: experimental research in New Zealand white rabbitsAlpaslan Öztürk1, Aysu Altıkardeşler İlman2, Hüsniye Sağlam3, Ulviye Yalçınkaya4, Serkan Aykut1, Semra Akgöz5, Yüksel Özkan1, Kemal Yanık2, Bijen Kıvçak3, Nazan Yalçın1, Recai Mehmet Özdemir11Department Of Orthopaedics And Traumatology, Bursa High Speciality, Research And Training Hospital, Bursa2Department Of Surgery, Veterinary Faculty, Uludag University, Bursa 3Faculty Of Pharmacy, Ege University, İzmir 4Department Of Pathology, Medical Faculty, Uludag University, Bursa, Turkey 5Department Of Biostatistics, Medical Faculty, Uludag University, Bursa, Turkey BACKGROUND: Phytoestrogens are plant-derived natural molecules having some bone forming and bone substituting effects. In the present study, the role of phytoestrogens on bone healing was investigated in a rabbit fracture model. METHODS: Twenty-two New Zealand white rabbits with right tibia fracture were divided into two groups randomly. The plant derived extract of Vitex agnus-castus L. (Verbenaceae) prepared before the study was administered intramuscularly in group 1 and group 2 was chosen as control. Fracture healing was monitored in weekly basis with blood alkaline phosphatase level, radiographs of extremities and 99m-Tc MDP bone scintigraphy. The study was finished at the end of the 3rd week. The extremities including tibial fractures were collected for histological examination. RESULTS: Radiographic evidence of fracture healing obtained on postoperative day seven was superior in group 1 than control group (p<0.01). The 99m-Tc MDP bone scintigraphy uptake ratios on postoperative seventh day showed higher uptake in group 1 than in group 2 (p<0.05). The differences of scintigraphic uptakes in fractured tibias calculated on postoperative seventh day and postoperative 14th in group 1 were higher than group 2 (p=0.04). The histopathologic evaluation performed after sacrification of all rabbits on postoperative 25th day showed no significant difference between both groups. No statistical difference was determined related to the other variables. CONCLUSION: Flavonoids affected positively the early periods of fracture healing mechanism in New Zealand white rabbits. We suggest further studies with phytoestrogens to determine the effects of various dosages and administration ways. Keywords: Experiment, flavonoids; fracture healing; scintigraphy.Alpaslan Öztürk, Aysu Altıkardeşler İlman, Hüsniye Sağlam, Ulviye Yalçınkaya, Serkan Aykut, Semra Akgöz, Yüksel Özkan, Kemal Yanık, Bijen Kıvçak, Nazan Yalçın, Recai Mehmet Özdemir. The effects of phytoestrogens on fracture healing: experimental research in New Zealand white rabbits. . 2008; 14(1): 21-27 Sorumlu Yazar: Alpaslan Öztürk, Türkiye |
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