Ulus Travma Acil Cerrahi Derg. 2007; 13(4): 274-280 | |||
İskemi reperfüzyon yaralanmasında resveratrol’ün iskelet kası üzerine etkileriNurzat Elmalı1, İrfan Esenkaya1, Neşe Karadağ2, Ferhat Taş1, Nevzat Elmalı31İnönü Üniversitesi, Tıp Fakültesi, Ortopedi Ve Travmatoloji Anabilim Dalı, Malatya2İnönü Üniversitesi, Tıp Fakültesi, Patoloji Anabilim Dalı, Malatya 3Sağlık Bakanlığı Okmeydanı Hastanesi, 5. Cerrahi Kliniği, İstanbul AMAÇ: Üzümde ve kırmızı şarapta bulunan polifenol yapıdaki resveratrol’ün çeşitli dokularda serbest radikal temizleyicisi ve antioksidan özelliklere sahip olduğu önceki çalışmalarda gösterilmiştir. Bu yazıda sıçanların arka ekstremitelerinde oluşturulan iskemi reperfüzyon (I/R) yaralanmasında resveratrol’ün kas dokusundaki etkileri araştırıldı. Effects of resveratrol on skeletal muscle in ischemia-reperfusion injuryNurzat Elmalı1, İrfan Esenkaya1, Neşe Karadağ2, Ferhat Taş1, Nevzat Elmalı31Department Of Orthopaedics And Traumatology, Medicine Faculty Of Inonu University, Malatya, Turkey2Departments Of Pathology, Medicine Faculty Of Inonu University, Malatya, Turkey 335th Department Of Surgery, Okmeydanı State Hospital, İstanbul, Turkey BACKGROUND: Resveratrol, a polyphenol found in grape and red wine, was previously shown to have free radical scavenging and antioxidant properties in various tissues. In this study, the effects of resveratrol were investigated in muscle tissue concerning the ischemia reperfusion (I/R) injury of rat hindlimb. METHODS: Arterial circulation of right hindlimbs of 24 Sprague-Dawley rats was ceased by a tourniquet applied for four hours (h). The tourniquet was released at the end of 4th hours and rats were divided into four groups of six rats. Then, extremity was reperfused for 4h in group I and for 8h in group II. Resveratrol in 0.5% ethyl alcohol was administered with a dose of 10 mg/kg in the treatment groups (group I and group II) intraperitoneally. Only 0.5% ethyl alcohol were administered in the control groups (group III and group IV) intraperitoneally. Gastrocnemius muscle was used for histological assessments and the anterior tibial muscle was used for measurement of malondialdehyde (MDA) levels. RESULTS: MN infiltration, edema, changes in diameters of muscle fibers and segmental necrosis were less prominent in rats treated with resveratrol compared with control groups (p<0.05). The MDA levels was significantly lower in treatment groups (p<0.05). CONCLUSION: The results suggest that resveratrol may protect the skeletal muscles against I/R injury with its potent antioxidant properties. Keywords: Antioxidants, free radicals; ischemia; rats, Sprague-Dawley; reperfusion; resveratrol; skeletal muscle.Nurzat Elmalı, İrfan Esenkaya, Neşe Karadağ, Ferhat Taş, Nevzat Elmalı. Effects of resveratrol on skeletal muscle in ischemia-reperfusion injury. Ulus Travma Acil Cerrahi Derg. 2007; 13(4): 274-280 Sorumlu Yazar: Nurzat Elmalı, Türkiye |
|